Microarray for molecular typing of Salmonella enterica serovars.
Identifieur interne : 002968 ( Main/Exploration ); précédent : 002967; suivant : 002969Microarray for molecular typing of Salmonella enterica serovars.
Auteurs : Joy Scaria [États-Unis] ; Raghavan U M. Palaniappan ; David Chiu ; Julie Ann Phan ; Lalit Ponnala ; Patrick Mcdonough ; Yrjo T. Grohn ; Steffen Porwollik ; Michael Mcclelland ; Chien-Shun Chiou ; Chishih Chu ; Yung-Fu ChangSource :
- Molecular and cellular probes [ 0890-8508 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , classification : DNA Probes.
- chemical , genetics : DNA Probes.
- genetics : Salmonella enterica.
- methods : Oligonucleotide Array Sequence Analysis.
- Cluster Analysis, Substrate Specificity.
Abstract
We describe the development of a spotted array for the delineation of the most common 14 disease-causing Salmonella serovars in the United States. Our array consists of 414 70 mers targeting core genes of Salmonella enterica, subspecies I specific genes, fimbrial genes, pathogenicity islands, Gifsy elements and other variable genes. Using this array we were able to identify a unique gene presence/absence profile for each of the targeted serovar which was used as the serovar differentiating criteria. Based on this profile, we developed a Matlab programme that compares the profile of an unknown sample to all 14 reference serovar profiles and give out the closest serovar match. Since we have included probes targeting most of the virulence genes and variable genes in Salmonella, in addition to using for serovar detection this array could also be used for studying the virulence gene content and also for evaluating the genetic relation between different isolates of Salmonella.
DOI: 10.1016/j.mcp.2008.04.002
PubMed: 18554865
Affiliations:
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Le document en format XML
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Our array consists of 414 70 mers targeting core genes of Salmonella enterica, subspecies I specific genes, fimbrial genes, pathogenicity islands, Gifsy elements and other variable genes. Using this array we were able to identify a unique gene presence/absence profile for each of the targeted serovar which was used as the serovar differentiating criteria. Based on this profile, we developed a Matlab programme that compares the profile of an unknown sample to all 14 reference serovar profiles and give out the closest serovar match. Since we have included probes targeting most of the virulence genes and variable genes in Salmonella, in addition to using for serovar detection this array could also be used for studying the virulence gene content and also for evaluating the genetic relation between different isolates of Salmonella.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><noCountry><name sortKey="Chang, Yung Fu" sort="Chang, Yung Fu" uniqKey="Chang Y" first="Yung-Fu" last="Chang">Yung-Fu Chang</name><name sortKey="Chiou, Chien Shun" sort="Chiou, Chien Shun" uniqKey="Chiou C" first="Chien-Shun" last="Chiou">Chien-Shun Chiou</name><name sortKey="Chiu, David" sort="Chiu, David" uniqKey="Chiu D" first="David" last="Chiu">David Chiu</name><name sortKey="Chu, Chishih" sort="Chu, Chishih" uniqKey="Chu C" first="Chishih" last="Chu">Chishih Chu</name><name sortKey="Grohn, Yrjo T" sort="Grohn, Yrjo T" uniqKey="Grohn Y" first="Yrjo T" last="Grohn">Yrjo T. Grohn</name><name sortKey="Mcclelland, Michael" sort="Mcclelland, Michael" uniqKey="Mcclelland M" first="Michael" last="Mcclelland">Michael Mcclelland</name><name sortKey="Mcdonough, Patrick" sort="Mcdonough, Patrick" uniqKey="Mcdonough P" first="Patrick" last="Mcdonough">Patrick Mcdonough</name><name sortKey="Palaniappan, Raghavan U M" sort="Palaniappan, Raghavan U M" uniqKey="Palaniappan R" first="Raghavan U M" last="Palaniappan">Raghavan U M. Palaniappan</name><name sortKey="Phan, Julie Ann" sort="Phan, Julie Ann" uniqKey="Phan J" first="Julie Ann" last="Phan">Julie Ann Phan</name><name sortKey="Ponnala, Lalit" sort="Ponnala, Lalit" uniqKey="Ponnala L" first="Lalit" last="Ponnala">Lalit Ponnala</name><name sortKey="Porwollik, Steffen" sort="Porwollik, Steffen" uniqKey="Porwollik S" first="Steffen" last="Porwollik">Steffen Porwollik</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Scaria, Joy" sort="Scaria, Joy" uniqKey="Scaria J" first="Joy" last="Scaria">Joy Scaria</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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